Jordan Holt

Immunofluorescence For immunostaining in 384 well plates, cells were fixed by adding and equal volume of 8% formaldehyde in PBS to media in wells to give a final concentration of 4% formaldehyde. Cells were fixed for 15 minutes at RT and washed three times with PBS. Permeabilization in PBS 0.5% Triton X-100 for 15 min was followed by blocking in 2% BSA in PBS (blocking buffer) for 1 h.