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Karim Ben M'Barek on Muck Rack

Karim Ben M'Barek

As seen in: JoVE- Journal of Visualized Experiments, Cold Spring Harbor Perspectives in Medicine (Online)
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Articles

Photoreceptor Cell Replacement Using Pluripotent Stem Cells: Current Knowledge and Remaining Questions

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By Olivier Goureau, Karim Ben M'Barek, Université d'Evry, Christelle Monville
| Cold Spring Harbor Perspectives in Medicine (Online)
Christelle Monville1,2, Olivier Goureau3 and Karim Ben M'Barek1,2,4 1INSERM U861, I-Stem, AFM, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases, 91100 Corbeil-Essonnes, France 2Université Paris-Saclay, Université d'Evry, U861, 91100 Corbeil-Essonnes, France 3Sorbonne Université, Institut de la Vision, INSERM, CNRS, 75012 Paris, France 4Centre d'Etude des Cellules Souches, 91100 Corbeil-Essonnes, France Correspondence: cmonville{at}istem.fr; olivier.goureau{at}inserm.fr;...
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Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

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By Walter Habeler, Alexandra Plancheron, Mohamed Jarraya, Karim Ben M'Barek
| JoVE- Journal of Visualized Experiments
hAMs contain an epithelial layer that should be removed before the seeding of RPE cells. An enzymatic treatment of the membrane is performed with the thermolysin under shaking. In order not to not lose the polarity of the membrane (the epithelium is on one side), it is fixed on a support which composition could be different depending on the provider (Figure 1A). Check the adhesion of the membrane to its support at this step and add clips if necessary.
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