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Kees Jalink

As seen in: BioRxiv, Cell Press, Cell Reports
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Articles by Kees Jalink

SRBD1, a highly conserved gene required for chromosome individualization

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By Jonne Raaijmakers, Louise M.E. Janssen, Abdelghani Mazouzi, Amber L.H. Hondema, Razvan Borza, Alexander Fish, Ahmed M.O. Elbatsh, Justina Kazokaitė-Adomaitienė, Nuria Vaquero-Siguero, Isabel Mayayo-Peralta, Leila Nahidiazar, Anoek Friskes, Liesbeth Hoekman, Onno B. Bleijerveld, Claire Hoencamp, Sarah Moser, Jos Jonkers, Kees Jalink, Wilbert Zwart, Patrick Celie, Benjamin D. Rowland, Anastassis Perrakis, Thijn R. Brummelkamp, René H. Medema
| Cell Reports Verified
Keywords SRBD1 condensin topoisomerase II chromosome individualization decatenation Research topic(s) CP: Molecular biology Introduction Cell viability is dependent on several key processes, such as translation, mRNA processing, intracellular transport, and cell division. Consequently, key factors that are required for the function of tRNAs, ribosomes, and chaperones are commonly essential for cell viability (DepMap portal1).
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Cytosolic Enhanced Dark Epac-Based FRET Sensors for Intracellular cAMP detection in live cells via FLIM

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By Giulia Zanetti, Jeffrey Klarenbeek, Kees Jalink
| BioRxiv
Abstract Fluorescence Resonance Energy Transfer (FRET)-based biosensors are powerful tools for studying second messengers with high temporal and spatial resolution. FRET is commonly detected by ratio-imaging, but Fluorescence Lifetime Imaging Microscopy (FLIM), which measures the donor fluorophore's lifetime, offers a robust and more quantitative alternative.
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Radical differences between two FLIM microscopes affect interpretation of cell signaling dynamics

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By Jeffrey Klarenbeek, Farid El Oualid, Kees Jalink, Sravasti Mukherjee
| BioRxiv
Abstract Emerging evidence suggests that cell signaling outcomes depend not only on the signal strength but also on its temporal progression. Our lab employs Fluorescence Lifetime Imaging of Resonance Energy Transfer (FLIM/FRET) biosensors to study intracellular signaling dynamics.
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By Elisa Matas-Rico, Michiel van Veen, Daniela Leyton-Puig, Jan Koster, Katarzyna M. Kedziora, Bas Molenaar, Marjolein J. A. Weerts, René H. Medema, Ben N. G. Giepmans, Anastassis Perrakis, Kees Jalink, Rogier Versteeg
| secure.jbs.elsevierhealth.com
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By Jan Koster, Katarzyna M. Kedziora, Kees Jalink, Elisa Matas-Rico, Michiel van Veen, Daniela Leyton-Puig, Bas Molenaar, Marjolein J. A. Weerts, René H. Medema, Ben N. G. Giepmans, Anastassis Perrakis, Rogier Versteeg
| secure.jbs.elsevierhealth.com
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Optotaxis: Caged Lysophosphatidic Acid Enables Optical Control of a Chemotactic Gradient

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By Felix Hövelmann, Katarzyna M. Kedziora, André Nadler, Rainer Müller, Kees Jalink
| Cell Press Verified
FiguresFigure 1Downstream Effects of LPA(A) Overview of LPA signaling.(B) LPA-induced calcium transients in N1E-115 neuroblastoma cells. Cells were loaded with the calcium indicator Oregon green BAPTA and stimulated by brief illumination with 405 nm light in the presence of 1 μM cgLPA. No desensitization of the cells was observed. Final calibration was achieved by adding 5 μM ionomycin and 5 mM calcium.(B′) N1E-115 stimulated by adding 1 μM of LPA to the medium.
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