Kenichiro Taniguchi

Human embryonic stem cell line H9 was used in this study (WA09, P30, P48, WiCell; NIH registration number: 0062). All hPSC lines were maintained in a feeder-free system for at least 20 passages and authenticated as karyotypically normal at the indicated passage number. Karyotype analysis was performed at Cell Line Genetics. All hPSC lines tested negative for mycoplasma contamination (LookOut Mycoplasma PCR Detection Kit, Sigma-Aldrich).

1 INTRODUCTION The diagnosis of conversion paralysis (CP) or hysterical paralysis (HP) is sometimes challenging as a diagnosis of exclusion for the clinician.1-4 The prevalence of patients with conversion palsy admitted to spinal cord units has been noted to be 0.3%–3.8%.2, 5-8 Although women are often misdiagnosed with CP, they may have genuine spinal cord lesions.1 In such cases, failure to “diagnose and treat,” especially in a timely manner, can result in an irreversible neurological...

We recently developed a biomimetic 3D culture system to induce the development of human amniotic ectoderm-like tissue from hPSC in vitro11. In this system, hPSC are plated as single cells at 30,000?cells?cm?2 onto a thick, soft gel bed of GeltrexTM, in mTeSR1 medium supplemented with 4% (v/v) GeltrexTM to establish a 3D extracellular environment (see Methods).