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Kum-Loong Boon

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In Vivo Mapping of Eukaryotic RNA Interactomes Reveals Principles of Higher-Order Organization and Regulation

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By Yang Shen, Miao Sun, Andreas Wilm, Kum-Loong Boon, Sidika Tapsin
| Cell Press Verified
FiguresFigure 1SPLASH Strategy Identifies RNA Interactions Accurately(A) Schematic of the SPLASH strategy. RNA-RNA interactions are crosslinked in vivo using biotinylated psoralen (biopsoralen) and then fragmented. Interacting regions that contain biopsoralen are enriched by binding to streptavidin beads and ligated together. Chimeric RNAs are then cloned into a cDNA library for deep sequencing.(B) Visualization of chimeric interactions on the secondary structure of the 28S rRNA.
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