Leo Shen

PUBLISHED June 10th, 2023 08:00 am FacebookTweetPinEmailLinkedIn Encircling the striking Masjid Sultan mosque and just a stone’s throw from popular indie movie theatre The Projector, Haji Lane in Kampong Glam is bursting with creative and colourful energy that can be felt everywhere within it.

Cell culture HEK293T cells (ATCC CRL-3216) and K562 cells (ATCC CCL-243) were cultured at 37 °C under 5% CO2 in DMEM (Corning) supplemented with 10% FBS (Clontech), 100 units per ml of penicillin and 100 µg ml−1 streptomycin (DMEM complete). Cells were tested every month for mycoplasma. A human iPSC (hiPSC), the WTC-11 cell line (GM25256, Coriell Institute) (Kreitzer et al., 2013), was used for all iPSC experiments in this study.

Rv1747 Regulatory Module Is Composed of Independent FHA-1 and FHA-2 Domains Joined by a Disordered Linker FHA-1 Has a Canonical FHA Domain Topology whereas FHA-2 Is Circularly Permuted FHA-2 Is More Dynamic and Less Stable than FHA-1 FHA-1 and FHA-2 Bind the Phosphorylated Linker to Form Inter- and Intramolecular Complexes The More Dynamic FHA-2 Has Higher Affinity for Phosphopeptides Protein Expression and Purification of Rv1747 Constructs for NMR Spectroscopy Protein Expression and...