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2. Materials and methods 2.1 Study design The experiments were not randomised. No statistical methods were used to determine the sample size. The researchers were not blinded to the distribution during the experiments and outcome analyses. 2.2 Single-cell RNA sequencing (scRNA-seq) data retrieval and isolation of human articular chondrocytes [26] Ji Q. et al. Single-cell RNA-seq analysis reveals the progression of human osteoarthritis. Ann. Rheum. Dis. 78: 100-110 ].
Supplementary Materials Supplementary Table 1. Supplementary Table 2. Primer sequence used for qRT-PCR Supplementary Figure 1. Effect of PTU-induced prolonged neonatal hypothyroidism on thyroids in rats aged 49 days. (A, B) Classical images of thyroid glands of WT and hypothyroid (Hypo) rats for different genders; scale bar, 0.2 cm. A representative image is shown from three female and three male rats. Hypertrophy of thyroid glands was observed in both female and male Hypo rats.
Introduction The knee joint has a complicated microenvironment consisting of many types of cells, such as osteocytes, hematopoietic stem cells, chondrocytes, synovial cells, nerve cells, and fat cells. Osteoarthritis (OA) is a progressive and heterogeneous disease that contributes to articular cartilage degeneration, alterations in subchondral bone, and synovial hyperplasia (Allen and Golightly, 2015; Martel-Pelletier et al., 2016).
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