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Lingci Huang on Muck Rack

Lingci Huang

As seen in: The Journal of Biological Chemistry
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Articles

mRNA translational dynamics mediates totipotent-like reprogramming

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By Jun Zhang, Xinwei Wu, Lingci Huang
| The Journal of Biological Chemistry Verified
Materials and methods ESCs were cultured on plates coated with 0.2% gelatin in KnockOut™ DMEM (Gibco) supplemented with 15% KnockOut™ Serum Replacement (Gibco), 1% non-essential amino acids (Gibco), 1% GlutaMAX™ (Gibco), 1% penicillin-streptomycin (Life Technologies), 0.1 mM β-mercaptoethanol (Invitrogen), and 1000 U/mL recombinant mouse leukemia inhibitory factor (LIF; Millipore). Cultures were maintained at 37 °C in a humidified atmosphere of 5% CO2.
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