Mangyu Choe

Get full text access Log in, subscribe or purchase for full access. References 1. DeBerardinis, R.J. ∙ Chandel, N.S. We need to talk about the Warburg effect Nat. Metab. 2020; 2:127-129 2. King, M.P. ∙ Attardi, G. Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation Science. 1989; 246:500-503 3. Titov, D.V. ∙ Cracan, V. ... Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio Science. 2016; 352:231-235 4. Sacktor, B.

Abstract Glycolysis is a conserved metabolic pathway that produces ATP and biosynthetic precursors. Here, we use mathematical modeling to investigate how the control of mammalian glycolytic enzymes through allostery and mass action accomplishes various tasks of ATP homeostasis, such as controlling the rate of ATP production, maintaining high and stable ATP levels, and ensuring that ATP hydrolysis generates a net excess of energy.

Abstract Mitochondrial membrane potential (ΔΨm) is one of the key parameters controlling cellular bioenergetics. Investigation of the role of ΔΨm in live cells is complicated by a lack of tools for its direct manipulation without off-target effects. Here, we adopted the uncoupling protein UCP1 from brown adipocytes as a genetically encoded tool for direct manipulation of ΔΨm. We validated the ability of exogenously expressed UCP1 to induce uncoupled respiration and lower ΔΨm in mammalian cells.