Maria J. Barbosa

Keywords Phaeodactylum tricornutum CRISPR Cas12a NHEJ HDR Introduction Diatoms are ubiquitous photosynthetic microorganisms that play a significant role in global ecosystems by providing 20% of the world’s primary production via CO2 fixation [1]. Within this group, the marine diatom Phaeodactylum tricornutum has emerged as a pivotal model organism for fundamental research and biotechnology applications. In particular, P.

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Trophic modes and light supply for production Microalgae are commonly grown by exploiting their photoautotrophic capacity in which cells harvest light energy, use carbon dioxide (CO2) as a carbon source, and release oxygen (O2) as a by-product. Alternatives to autotrophic cultures are heterotrophic cultures in which organic carbon, such as sugars and organic acids, are used as carbon sources in the absence of light.