Marie-Ming Aynaud

The authors have declared no competing interest. This work is supported by Canadian Institutes of Health Research (Grant #177705) I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained. Yes The details of the IRB/oversight body that provided approval or exemption for the research described are given below: The Mount Sinai Hospital Research Ethics Board in Toronto, Canada gave ethical approval for this work.

Highlights • Time-resolved single-cell RNA-seq of EWSR1-FLI1 induction in an Ewing sarcoma cell line • Highly specific transcriptomic signature of EWSR1-FLI1 chimeric oncogene • Sources of transcriptomic heterogeneity in Ewing tumors • Correlations between EWSR1-FLI1 activity and metabolic and other phenotypic states Summary EWSR1-FLI1, the chimeric oncogene specific for Ewing sarcoma (EwS), induces a cascade of signaling events leading to cell transformation.

New Results View ORCID ProfileJulian Musa, View ORCID ProfileFlorencia Cidre-Aranaz, Marie-Ming Aynaud, View ORCID ProfileMartin F. Orth, Olivier Mirabeau, Mor Varon, Sandrine Grossetete, Didier Surdez, View ORCID ProfileShunya Ohmura, View ORCID ProfileJulia S Gerke, View ORCID ProfileAruna Marchetto, View ORCID ProfileMarlene Dallmayer, View ORCID ProfileMichaela C. Baldauf, Moritz Gartlgruber, Frank Westermann, Stefanie Stein, View ORCID ProfileTilman L. B.