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Abstract Clostridium botulinum causes the disease botulism, and nontoxigenic Clostridium sporogenes is closely related to group I C. botulinum. Despite its pathogenicity, C. botulinum remains poorly characterized. Genetic manipulation is critical for understanding bacterial physiology and disease. We compared the conjugal transformation efficiencies of seven strains, including group I C. botulinum (strains 62A, 7I03-H, Okra, Osaka05, 111) and C.
Mini-HA (#1) is observed to binds to E-cadherin with a dissociation constant (K D ) similar to that of HA (∼2.7 μM vs . ∼2.3 μM) ( 14 Lee K. Zhong X. Gu S. Kruel A.M. Dorner M.B. Perry K. Rummel A. Dong M. Jin R. Molecular basis for disruption of E-cadherin adhesion by botulinum neurotoxin A complex. ), and disrupts the epithelial barrier ( Fig. 1 B) ( 14 Lee K. Zhong X. Gu S. Kruel A.M. Dorner M.B. Perry K. Rummel A. Dong M. Jin R.
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