Abstract Regenerative agriculture can be deconstructed into several constituent practices, including adaptive multi-paddock (AMP) grazing, improved biodiversity, silvopasture, and minimizing cultivation and synthetic fertilizer inputs. Here, using farms across a rainfall gradient, we examined how three constituents—pasture species composition, antecedent soil organic carbon (SOC) and AMP grazing—influenced SOC accrual, greenhouse gas (GHG) emissions, production and profitability.