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M.G. Bartlett

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Impact of column selection for the analysis of 3mer phosphorothioated oligonucleotides in hydrophilic interaction liquid chromatography

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By A.J. Alpert, U.D. Neue, M.G. Bartlett
| ScienceDirect Verified
Introduction The development of therapeutic oligonucleotides (ONs) for gene and antisense therapy has been progressing in recent years [1]. With increasing complexity of ONs due to their chemical modifications, the demand for reliable and robust analytical methods for therapeutic ONs is increasing [2]. The first approved ON drug (fomivirsen, 1998) and the following therapeutic ONs commonly use phosphorothioate (PS) modification.
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