Michael Bartoschek

Abstract Large genes including several CRISPR-Cas modules like gene activators (CRISPRa) require dual adeno-associated viral (AAV) vectors for an efficient in vivo delivery and expression. Current dual AAV vector approaches have important limitations, e.g., low reconstitution efficiency, production of alien proteins, or low flexibility in split site selection. Here, we present a dual AAV vector technology based on reconstitution via mRNA trans-splicing (REVeRT).

Correction to: Nature Communications https://doi.org/10.1038/s41467-020-19603-1, published online 24 November 2020. The original version of this Article contained an error in the spelling of the author Makoto Nakanishi, which was incorrectly given as Makoto Nakanashi. This has now been corrected in both the PDF and HTML versions of the Article. Author information Author notes These authors contributed equally: Atsuya Nishiyama, Joel Ryan.

Abstract DNA nanotechnology is an emerging field that promises fascinating opportunities for the manipulation and imaging of proteins on a cell surface. The key to progress is the ability to create a nucleic acid–protein junction in the context of living cells. Here we report a covalent labelling reaction that installs a biostable peptide nucleic acid (PNA) tag. The reaction proceeds within minutes and is specific for proteins carrying a 2 kDa coiled-coil peptide tag.