Michael Rourke

At the outset, our approach to engineering was to generate many diverse variants of CoCYP119 that are all compatible with MHAT, and could be screened for new modes of reactivity. Serendipitously, color changes during screening for deallylation with 1a suggested that a new MHAT reaction had already emerged ( Figure S18 ). LC-MS analysis indicated that, in addition to the expected deallylation product ( 1b ), a new product was forming with +2 amu relative to 2,4-dinitrophenolate 1b ( Figure S19 ).

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