Michelle Grace Acoba

Fig 7. Pbp1 associates with mRNAs through Puf3. A. Strains expressing Pbp1-Flag, Puf3-HA or Puf3-Flag, Pbp1-HA were grown in YPD or switched to YPL for 3 h. Flag immunoprecipitation of Pbp1-Flag or Puf3-Flag was performed, and the beads were treated with or without RNase If for 15 min to assess whether the associating amount of Puf3-HA or Pbp1-HA is impacted by the presence of any bound RNA. B. RNA IP analysis of transcripts bound to either Pbp1 or Puf3.

Highlights • SFXNs are atypical TIM22 complex substrates • Loss of SFXN1 leads to respiratory chain impairments, most damaging to complex III • SFXN1 contributes to coenzyme Q, heme, α-ketoglutarate, and one-carbon metabolism • Metabolite supplementation improves complex III function in the absence of SFXN1 Summary Mitochondrial carriers (MCs) mediate the passage of small molecules across the inner mitochondrial membrane (IMM), enabling regulated crosstalk between compartmentalized reactions.