Montagnani Marelli

Tasnim Mohamed† Alessandra Colciago† Marina Montagnani Marelli Roberta Manuela Moretti Valerio Magnaghi* Department of Pharmacological and Biomolecular Sciences “Rodolfo Paoletti”, University of Milan, Milan, Italy Introduction: Protein kinase type C-ε (PKCε) plays an important role in the sensitization of primary afferent nociceptors, promoting mechanical hyperalgesia.

3. Discussion DTX is the first-line therapy for patients with metastatic CRPC, but acquisition of drug resistance due to evasion and defection of apoptosis often leads to the failure of this therapeutical approach [21]. Natural products are emerging as a promising source for effective anticancer agents and are often less toxic than chemotherapeutic agents. Additionally, they can activate molecular mechanisms able to contrast resistance to therapies [44].

BLM and A375 cells, or normal human melanocytes, were treated with δ-TT (5–20 μg/ml) for 24 h or 48 h; cell viability was assessed by MTT assay. Figure 1a shows that the tocotrienol dose-dependently inhibited the number of viable melanoma cells at both time intervals, being significantly effective at 10–20 μg/ml (Fig. 1a). These doses correspond to those previously reported for the antitumor activity of tocotrienols in cancer cell lines33,34,35.