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The spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer

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By Evan Paul Verified, Inc. Rockville, MultiplexDx s.r.o, Barbora Huraiova
| tu-dresden.de
Current assays fail to address breast cancer's complex biology and accurately predict treatment response. On a retrospective cohort of 1082 female breast tissues, we develop and validate mFISHseq, which integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser capture microdissection. This technique ensures tumor purity, unbiased whole transcriptome profiling, and explicitly quantifies intratumoral heterogeneity.
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