Muneef Ayyash

Highlights • Inhibition of SRC, PKC, and WNT consolidates human naive pluripotency induction • Competitiveness of p53 depleted human PSCs in cross-species chimeric embryos • Opposing net effect for ACTIVIN and WNT on mouse versus human naive pluripotency • 2i and ERKi independent alternative human naive-like PSC conditions Summary Isolating human MEK/ERK signaling-independent pluripotent stem cells (PSCs) with naive pluripotency characteristics while maintaining differentiation competence and...

Tripartite Inhibition of SRC-WNT-PKC Signalling Consolidates Human Naive Pluripotency Jonathan Bayerl, Muneef Ayyash, Tom Shani, Yair Manor, Ohad Gafni, Yael Kalma, Alejandro Aguilera-Castrejon, Mirie Zerbib, Hadar Amir, Daoud Sheban, Shay Geula, Nofar Mor, Leehee Weinberger, Vladislav Krupalnik, Bernardo Oldak, Nir Livnat, Shadi Tarazi, Shadi Tawil, Lior Lasman, Suhair Hanna, Noa Novershtern, Dalit Ben-Yosef, Sergey Viukov, Jacob H.

Drug development is currently hampered by the inability of animal experiments to accurately predict human response. While emerging organ on chip technology offers to reduce risk using microfluidic models of human tissues, the technology still relies on end-point assays to assess tissue damage resulting in limited mechanistic information and an inability to detect adverse effects occurring at sub-toxic drug exposure.