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Articles

Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration

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By Hiroyuki Katayama, Hiroshi Hama, Koji Nagasawa, Hiroshi Kurokawa, Mayu Sugiyama, Megumu Takahashi, Yoko Ishida, Hiroyuki Hioki, Atsushi Miyawaki, Ryoko Ando, Masaaki Funata, Nobuyo Yoshida, Misaki Homma, Takanori Nishimura, Yoshiyuki Tsujihata
| ScienceDirect Verified
Highlights • mito-SRAI provides reliable mitophagy readouts under both live and fixed conditions • mito-SRAI uses a fluorescent protein that is resistant to lysosomal environments • High-throughput screening led to the discovery of mitophagy-inducing compounds • Evidence was given for loss of nigral dopaminergic neurons due to mitophagy failure Summary Dysfunctional mitochondria accumulate in many human diseases.
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