Skip To Main Content
Olga Timoshenko on Muck Rack

Olga Timoshenko

As seen in: MDPI

Get in touch with Olga

Contact Olga, search articles and posts on X, monitor coverage, and track replies from one place.

Learn more about Muck Rack

Actions

Share this page

Articles

Combining Asymmetric PCR with PAM-Independent Cas12a Analysis of Single-Stranded DNA Amplicons for Programmable Biosensing: Application to Pectobacterium polaris Detection

|
By Konstantin G. Ptitsyn, Olga Timoshenko, Svetlana A. Khmeleva
| Preprints.org
You are already at the latest version A programmable Cas12a-based biosensing system is developed for sensitive and se-lective detection of Pectobacterium polaris, a newly emerging pathogen affecting a wide range of agriculturally important plants. The system relies on asymmetric PCR (aPCR) and PAM-independent recognition of single-stranded DNA by a Cas12a/gRNA complex and allowed to determine P. polaris with limit of detection of 10 copies of bacterial genome per reaction.
Open in Who Shared Issue with article?

Detection of Potato Pathogen Clavibacter sepedonicus by CRISPR/Cas13a Analysis of NASBA Amplicons

|
By Svetlana A. Khmeleva, Leonid K. Kurbatov, Konstantin G. Ptitsyn, Olga Timoshenko
| MDPI
All articles published by MDPI are made immediately available worldwide under an open access license. No special permission is required to reuse all or part of the article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. For more information, please refer to https://www.mdpi.com/openaccess.
Open in Who Shared Issue with article?

Effects of Isoxazolyl Steroids on Key Genes of Sonic Hedgehog Cascade Expression in Tumor Cells

|
By Anna Aleksandrova, Arif R. Mekhtiev, Olga Timoshenko, Elena Kugaevskaya
| MDPI
All articles published by MDPI are made immediately available worldwide under an open access license. No special permission is required to reuse all or part of the article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. For more information, please refer to https://www.mdpi.com/openaccess.
Open in Who Shared Issue with article?
See all 4 articles