Ondřej Peterka

Abstract Pancreatic cancer is one of the most aggressive malignancies with a 5-year relative survival rate of only 13%. Its poor prognosis is largely attributed to the lack of reliable tools for early detection. Current diagnostic standards rely on imaging methods that are invasive, costly, and often inadequate to detect early-stage disease. A noninvasive blood-based test with high sensitivity and specificity could substantially improve patient outcomes.

Extraction of Less Polar Lipids The main objective of this study was to develop a selective and efficient workflow for the extraction and analysis of less polar lipid classes from human plasma. Although these lipid species can be analyzed by using commonly applied chromatographic techniques, such approaches often introduce significant limitations when applied to this lipid group.

Introduction Click to copy section linkSection link copied! Lipidomics is a field dealing with the determination of lipids in biological systems at a defined time. Within this complex landscape, a group of lipids centered around a glycerol or sterol backbone plays fundamental roles in energy storage, membrane structure, and signaling pathways.