Outa Nakashima on Muck Rack

Outa Nakashima

As seen in: Cell Press
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Articles

Mapping intracellular dynamics across the whole cell with spatial statistics

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By Yohei Okabe, Takumi Saito, Outa Nakashima, Daiki Matsunaga, Shinji Deguchi
| Cell Press Verified
ArticleOnline nowOpen access 1Graduate School of Engineering Science, The University of Osaka 2R3 Institute for Newly-Emerging Science Design, The University of Osaka 3Global Center for Medical Engineering and Informatics, The University of Osaka Publication History: Received February 8, 2025; Accepted October 3, 2025; Published online October 7, 2025 DOI: 10.1016/j.bpj.2025.10.005 External LinkAlso available on ScienceDirect External Link Copyright: © 2025 The Author(s).
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Mapping molecular diffusion across the whole cell with spatial statistics-based FRAP

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By Takumi Saito, Outa Nakashima, Daiki Matsunaga, Yohei Okabe
| biorxiv.org
Abstract Understanding molecular diffusion within cells is crucial for gaining insights into cellular biophysical mechanisms. Fluorescence recovery after photobleaching (FRAP) is a powerful technique for assessing molecular diffusion, yet its localized measurement approach hinders whole-cell analysis.
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Decoupling diffusion, turnover, and advection in long-term FRAP

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By Takumi Saito, Shinji Deguchi, Outa Nakashima
| biorxiv.org
Abstract Intracellular molecular turnover is a dynamic process governed by diffusion, biochemical reactions, and intracellular transport dynamics. While fluorescence recovery after photobleaching (FRAP) has been widely used to quantify fluorescence recovery mechanisms, conventional models primarily focus on diffusion and reaction kinetics, often overlooking the influence of intracellular advection.
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