Panneerselvam Jayabal

(A) NOS2, NOS3, and SFRP2 silencing by siRNA transfection. RB383 cells were transfected with the indicated siRNAs, and the RNA levels of NOS2, NOS3, and SFRP2 were assessed by qRT-PCR 12 h after transfection (n = 3). (B) NOS2 silencing abrogates NO production following SFRP2 silencing. RB383 cells were transfected with the indicated siRNAs, and the production of NO was assessed by nitrite/nitrate calorimetric assays of the CM 12 h after transfection (n = 3).

Highlights • The autocrine signaling mediated by NELL2 maintains Ewing sarcoma cell growth • NELL2 signaling inhibits cdc42 and enhances EWS-FLI1 transcriptional output • NELL2-cdc42 signaling regulates the assembly and the stability of BAF complexes • Ewing sarcoma harbors cell populations displaying high and low NELL2 signaling Summary BAF chromatin remodeling complexes play important roles in chromatin regulation and cancer.

Highlights • The autocrine signaling mediated by GDF6 maintains Ewing sarcoma growth • The GDF6 prodomain is a ligand for CD99, a widely used marker for Ewing sarcoma • GDF6 triggers CSK recruitment to CD99 intracellular domain, inhibiting Src activity • Klippel-Feil syndrome-linked GDF6 mutants are hyperactive in CD99-Src signaling Summary We report here that the autocrine signaling mediated by growth and differentiation factor 6 (GDF6), a member of the bone morphogenetic protein (BMP)...