Patrick Barendse

Abstract Protein–protein interactions are at the core of all key biological processes. However, the complexity of the structural features that determine protein–protein interactions makes their design challenging. Here we present BindCraft, an open-source and automated pipeline for de novo protein binder design with experimental success rates of 10–100%. BindCraft leverages the weights of AlphaFold2 (ref.

Keywords prokaryotic immunity host defense Argonaute Cas4 pAgo nuclease PD-(D/E)xK cyanobacteria Introduction Argonaute proteins (Agos) are present in all domains of life.1 Eukaryotic Argonautes (eAgos) are the key component of the RNA-induced silencing complex (RISC) in which eAgos use guide RNAs to target complementary RNA targets.2,3,4 As such, eAgos regulate gene expression, interfere with viral RNA, and silence transposon activity.

Abstract Anti-CRISPR proteins (Acrs) block the activity of CRISPR-associated (Cas) proteins, either by inhibiting DNA interference or by preventing crRNA loading and complex formation. Although the main use of Acrs in genome engineering applications is to lower the cleavage activity of Cas proteins, they can also be instrumental for various other CRISPR-based applications.