Paz Drori

Abstract Mainstream virus detection relies on the specific amplification of nucleic acids via polymerase chain reaction, a process that is slow and requires extensive laboratory expertise and equipment. Other modalities, such as antigen-based tests, allow much faster virus detection but have reduced sensitivity. In this study, we report the development of a flow virometer for the specific and rapid detection of single nanoparticles based on confocal microscopy.

Abstract Super-resolution light microscopy techniques facilitate the observation of nanometer-size biomolecules, which are 1-2 orders of magnitude smaller than the diffraction limit of light. Using super-resolution microscopy techniques it is possible to observe fluorescence from two biomolecules in close proximity, however not necessarily in direct interaction.

Highlights • trFRET-guided DMD simulations are used to study α-synuclein monomer conformations • Multifunctions of α-synuclein are explained by its monomeric structures • Millisecond conformational dynamics of α-synuclein monomer is discovered by smPIFE Summary α-Synuclein plays an important role in synaptic functions by interacting with synaptic vesicle membrane, while its oligomers and fibrils are associated with several neurodegenerative diseases.