Pradeep Reddy Cingaram

Abstract Precision nuclear run-on (PRO) sequencing (PRO-seq) is a powerful technique for mapping polymerase active sites with nucleotide resolution and measuring newly synthesized transcripts at both promoters and enhancer elements. The current PRO-seq protocol is time-intensive, technically challenging, and requires a large amount of starting material.

1 Introduction Breast cancer is the most prevalent type of tumor in women, affecting a significant number of them worldwide (Meer et al., 2021). Recent statistics estimate that breast cancer accounts for approximately 25% of all cancer cases diagnosed in women globally (Meer et al., 2021). Despite significant advances in the treatment of breast cancer, the management of this disease remains a considerable challenge. (Barrios, 2022).