Jingyin Yue

Keywords rapid PRO-seq nascent transcription RNA polymerase II transcriptional elongation transcriptional pausing enhancer RNA INTS11 Integrator complex hematopoietic progenitor cells neurodevelopmental genes Get full text access Log in, subscribe or purchase for full access. References 1. Fuda, N.J. ∙ Ardehali, M.B. ∙ Lis, J.T. Defining mechanisms that regulate RNA polymerase II transcription in vivo Nature. 2009; 461:186-192 2.

Flow cytometry Cell surface marker staining for HSPCs or differentiated populations in BM, spleen, thymus and fetal liver was performed as previously described (see also Table S2 for the abbreviations and cell surface markers of each sub populations in BM).44 Briefly, 1 to 10 million cells were suspended in 200ul PBS supplemented with 2% FBS and corresponding flow antibodies and incubated on ice for 30 min.

Abstract Precision nuclear run-on (PRO) sequencing (PRO-seq) is a powerful technique for mapping polymerase active sites with nucleotide resolution and measuring newly synthesized transcripts at both promoters and enhancer elements. The current PRO-seq protocol is time-intensive, technically challenging, and requires a large amount of starting material.