Puping Liang on Muck Rack

Puping Liang

As seen in: Nature, Journal of Banking Regulation
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Articles

Genome-wide profiling of adenine base editor specificity by EndoV-seq

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By Puping Liang, Xiaowei Xie, Hongwei Sun, Shengyao Zhi
| Nature Verified
Abstract The adenine base editor (ABE), capable of catalyzing A•T to G•C conversions, is an important gene editing toolbox. Here, we systematically evaluate genome-wide off-target deamination by ABEs using the EndoV-seq platform we developed. EndoV-seq utilizes Endonuclease V to nick the inosine-containing DNA strand of genomic DNA deaminated by ABE in vitro. The treated DNA is then whole-genome sequenced to identify off-target sites.
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Correction of β-thalassemia mutant by base editor in human embryos

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By Puping Liang, Sun Yat-sen, Chenhui Ding, Hongwei Sun
| link.springer.com
The explosive growth of human genomic data has revealed unprecedented numbers of disease-causing point mutations. Repairing such mutations may offer the best, and in some cases, only cure for genetic diseases. We and other groups have sought to correct disease mutant by combining CRISPR/Cas9 and homology directed repair (HDR) in human tripronulcear zygotes and diploid zygotes.
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By Puping Liang, Peng Tan, Lian He, Junjiu Huang, Rong-Fu Wang, Jun Cui, Meixin Chen, Qingcai Meng, Yunfei Qin, Yubin Zhou, Yongjun Chen
| secure.jbs.elsevierhealth.com
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