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Quigly Dragotakes

As seen in: Nature, MDPI, BioRxiv, medRxiv, eLife, JoVE- Journal of Visualized Experiments, The Journal of Immunology
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Articles

A method for authenticating the fidelity of Cryptococcus neoformans knockout collections

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By Ella Jacobs, Quigly Dragotakes, Madhura Kulkarni, Amanda Dziedzic
| BioRxiv
Abstract Gene knockout strain collections are important tools for discovery in microbiology. The only available genome-wide deletion collection for a human pathogenic fungus, Cryptococcus neoformans, is utilized widely for genetic studies. We uncovered mix-ups in the assembly of the commercially available C. neoformans deletion collection of ~6,000 unique strains acquired by our lab. While pursuing the characterization of a gene-of-interest, the corresponding deletion strain from the C.
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Commonalities and differences in the Cryptococcus neoformans response to ingestion by protozoal and mammalian phagocytic cells suggests host cell metabolism as a target for intracellular survival

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By Quigly Dragotakes, Ella Jacobs, Seth D. Greengo, Gracen Gerbig
| BioRxiv
Abstract The outcome of the interaction between Cryptococcus neoformans and infected hosts can be determined by whether the fungal cell survives ingestion by phagocytic cells. This applies to both unicellular and multicellular hosts such as amoeba and animals, respectively. Ingestion by phagocytic cells results in the formation of the cryptococcal phagosome but this structure has proved difficult to isolate.
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Avoided and Avoidable Deaths with the Use of COVID-19 Convalescent Plasma in Italy during the First Two Years of Pandemic

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By Massimo Franchini, Arturo Casadevall, Quigly Dragotakes, Daniele Focosi
| MDPI
All articles published by MDPI are made immediately available worldwide under an open access license. No special permission is required to reuse all or part of the article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. For more information, please refer to https://www.mdpi.com/openaccess.
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