Randy A. Bartels

Statistical estimation methods for localization microscopy (LM) estimate emitter locations using a parameterized statistical model presumed for the data, enabling derivations of estimators and theoretical accuracy bounds.

Spatial frequency modulation imaging (SPIFI) has previously been demonstrated in multiphoton modalities with resolution enhancement. When signal light levels are low enough, signals consist of discrete pulses: this is a photon-counting regime. By binning photon counts into discrete time bins, SPIFI signals can be built up by incrementing the photon counts over many modulation periods.

Abstract Coherent Raman microscopy is the method of choice for the label-free, real-time characterization of the chemical composition in biomedical samples. The common implementation relies on scanning two tightly focused laser beams across the sample, which frequently leads to sample damage and proves slow over large fields of view. The few existing wide-field techniques, for their part, feature a reduced lateral resolution and do not provide axial sectioning.