Rapolas Zilionis

Microfluidics has emerged as a pivotal technology with applications across biology, biochemistry, and biomedicine. One of the key features offered by microfluidics is the possibility of conducting high-throughput analyses on single cells or biomolecules within microreactors in the pico- to nanoliter volume range. (1) Among diverse microfluidic systems reported to date, droplet-based single-cell analytical methods have gained a particularly broad interest and utility.

Microfluidics has emerged as a pivotal technology with applications across biology, biochemistry, and biomedicine. One of the key features offered by microfluidics is the possibility of conducting high-throughput analyses on single cells or biomolecules within microreactors in the pico- to nanoliter volume range. (1) Among diverse microfluidic systems reported to date, droplet-based single-cell analytical methods have gained a particularly broad interest and utility.

Highlights • Human dendritic cell and monocyte subsets show one-to-one equivalence in mouse • Neutrophils exhibit tumor-associated phenotypes that are conserved across species • Myeloid subsets in patient blood only partially overlap with those in their tumors • Unique markers define myeloid cell subsets and associate with clinical prognosis Summary Tumor-infiltrating myeloid cells (TIMs) comprise monocytes, macrophages, dendritic cells, and neutrophils, and have emerged as key regulators of...