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Robert Barretto on Muck Rack

Robert Barretto

As seen in: The New England Journal of Medicine, Science Magazine
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Articles

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

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By Julia Joung, Ann E. Woolley, Michael Segel, Robert Barretto
| The New England Journal of Medicine Verified
To the Editor: CRISPR (clustered regularly interspaced short palindromic repeats)–based diagnostic tests1,2 collectively provide a nascent platform for the detection of viral and bacterial pathogens.
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Multiplex Genome Engineering Using CRISPR/Cas Systems

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By David Cox, F. Ann Ran, Shuailiang Lin, Robert Barretto
| Science Magazine Verified
Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells.
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