Romain Le Bars

1 Introduction Bacterial transcription terminates through two alternative pathways (Ray-Soni et al. 2016). Intrinsic (Rho-independent) termination requires no auxiliary factors and is dependent for the most part on the formation of a stable secondary structure in the nascent RNA. The second pathway depends on the conserved, ATP-driven termination factor Rho.

Abstract The nucleoid-associated protein H-NS is a bacterial xenogeneic silencer responsible for preventing costly expression of genes acquired through horizontal gene transfer. H-NS silences several Salmonella Pathogenicity Islands (SPIs) essential for host infection. The stochastic expression of SPI-1 is required for invasion of host epithelial cells but complicates investigation of factors involved in SPI-1 chromatin structure and regulation.

Method details Antibodies and cell reagents The rabbit polyclonal anti-P antibody was previously described 18 Lahaye X. Vidy A. Pomier C. Obiang L. Harper F. Gaudin Y. Blondel D. Functional characterization of Negri bodies (NBs) in rabies virus-infected cells: Evidence that NBs are sites of viral transcription and replication. and was used at a 1:2000 dilution for immunofluorescence (IF) staining and 1:1000 for western blot (WB) analysis.