Roman Barth

Keywords cohesin CTCF magnetic tweezers single-molecule fluorescence AlphaFold chromosome organization DNA loop extrusion Get full text access Log in, subscribe or purchase for full access. References 1. Davidson, I.F. ∙ Peters, J.M. Genome folding through loop extrusion by SMC complexes Nat. Rev. Mol. Cell Biol. 2021; 22:445-464 2. Kim, E. ∙ Barth, R. ∙ Dekker, C. Looping the Genome with SMC Complexes Annu. Rev. Biochem. 2023; 92:15-41 3. Davidson, I.F. ∙ Bauer, B. ∙ Goetz, D. ...

Abstract Cohesin extrudes DNA into loops and is positioned along the genome by stalling at CTCF upon encountering its N-terminal region (NTR). The mechanism underlying this stalling, however, is unresolved. Using single-molecule assays that monitor DNA loop extrusion (LE) in the presence of NTR fragments, we identify two amino acid motifs, YDF and KTYQR, that hinder LE.

Keywords SMC complexes DNA loop extrusion single molecule visualization cohesin NIPBL SMC5/6 condensin Introduction Genomes across the tree of life are organized and constantly reshaped by active processes within the cell. Structural maintenance of chromosomes (SMC) protein complexes are important, owing to their major role in chromosome organization.1 The three major members of the SMC family in eukaryotes are condensin, cohesin, and SMC5/6.