Shengying Li

1 Introduction Enzymatic DNA synthesis has garnered significant attention and achieved remarkable breakthroughs in the past decades [1-11]. This important enabling technology holds tremendous potential for a broad range of applications across diverse fields, including gene/genome synthesis [12-15], DNA-based data storage [16-21], DNA origami [22-25], medical diagnostics [26-32], and environmental monitoring [33-36].

Keywords polyethylene terephthalate machine learning enzymatic depolymerization MD simulation dual-enzyme system Introduction Among the petroleum-based plastics, polyethylene terephthalate (PET) is one of the most widely produced synthetic polymers, with a global manufacturing capacity exceeding 30 million tons annually. The extensive use of PET in packaging and textiles, combined with its recalcitrant nature, has led to pressing challenges in plastic waste management and environmental pollution.