Shu-Guang Wang

Abstract Prime editing enables versatile and precise genomic modifications, following a fundamental principle that the editing extends from the nick site towards the 3′ direction of the cleaved DNA strand. Here, we report the unknown competency of prime editing on the opposite 5′ direction of the nicked DNA strand through a hidden reverse transcription pathway, where a designed edit can be integrated upstream of the nick.

Abstract Base editing is preferable for bacterial gene inactivation without generating double strand breaks, requiring homology recombination or highly efficient DNA delivery capability. However, the potential of base editing is limited by the adjoined dependence on the editing window and protospacer adjacent motif (PAM). Herein, we report an unconstrained base editing system to enable the inactivation of any genes of interests (GOIs) in bacteria.

Cu(II) inhibited the transport of tetracycline in porous media: Role of complexation Tetracycline (TC) and Cu(II) coexist commonly in various waters, which may infiltrate into the subterranean environment through runoff and leaching, resulting in substantial ecological risks. However, the underlying mechanisms of why Cu(II) affects the transport of TC in porous media remain to be further explored and supported by more evidence, especially the role of complexation.