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TaCNGC-2A suppresses seed dormancy and activates pre-harvest sprouting through modulating calcium and hormonal signaling pathways
Abstract Weak seed dormancy (SD) is prone to pre-harvest sprouting (PHS), which reduces cereal yield and quality. Here, through map-based analysis, we identify TaCNGC-2A, encoding a cyclic nucleotide-gated channel protein, as a negative regulator of wheat SD. Knocking out of TaCNGC-2A enhances SD and PHS resistance, with no yield penalty. Two transcription factors, TaMYB-5B and TaMYB-5D, directly bind to the T/A mutation site of TaCNGC-2A promoter to synergistically repress its expression.
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