Silja Heilmann

Abstract During embryogenesis dynamic changes in tissue architecture transform primitive anlages to functional organs. Here we document in real time how pancreatic lumens are derived and transformed using a new apical-polarity mouse reporter. Our 4D imaging data reveals dynamic remodeling of apical proteins and lumens to primarily drive each stage of pancreatic duct development. Furthermore, we pinpoint two unique transitions during lumenogenesis.

Abstract During embryogenesis dynamic changes in tissue architecture transform primitive anlages to functional organs. Here we document in real time how pancreatic lumens are derived and transformed using a new apical-polarity mouse reporter. Our 4D imaging data reveals dynamic remodeling of apical proteins and lumens to primarily drive each stage of pancreatic duct development. Furthermore, we pinpoint two unique transitions during lumenogenesis.

Highlights • p120ctn expression segregates pancreatic progenitors into fate determining niches • p120ctn-low progenitors segregate into peripheral domains and form pro-acinar tips • p120ctn-high progenitors remain in the trunk to become duct and endocrine cells • p120ctn downregulation in endocrine cells drives delamination Summary The mechanism of how organ shape emerges and specifies cell fate is not understood.