Soha Salloum

Keywords mRNA localization cotranslational assembly polysome transport centrosomal mRNAs EJC HSP90/R2TP chaperone (PAQosome) Get full text access Log in, subscribe or purchase for full access. References 1. Cassella, L. ∙ Ephrussi, A. Subcellular spatial transcriptomics identifies three mechanistically different classes of localizing RNAs Nat. Commun. 2022; 13:6355 2. Zein-Sabatto, H. ... Centrocortin potentiates co-translational localization of its mRNA to the centrosome via dynein BioRxiv. 2024; 3.

Abstract The HSP90/R2TP quaternary chaperone assembles key cellular machines, including the three nuclear RNA polymerases and many non-coding RNPs. Here, we characterized the RNA associated to R2TP and found that it binds many partners co-translationally. Its co-translational interactome further reveals many novel potential clients and identifies clients bound only co-translationally, only post-translationally, or both.

Abstract β-catenin is a multi-functional protein playing essential roles in cancer. It bridges E-cadherin to the cytoskeleton and also activates transcription in response to Wnt. Plasma membrane β-catenin is stable whereas without Wnt, cytoplasmic β-catenin is degraded by the destruction complex, composed of APC and Axin. Here, we show that APC and Axin associate with many mRNAs and that this occurs via the nascent protein chains.