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Sulforaphane exerts anti-angiogenesis effects against hepatocellular carcinoma through inhibition of STAT3/HIF-1α/VEGF signalling
SFN was purchased from Toronto Research Chemicals (Toronto, Canada). Complete protease inhibitors were obtained from Roche Applied Science (West Sussex, UK). Primary antibodies to STAT3 (cat. no. 8019), p-STAT3 (cat. no. 8059), ?-actin (cat. no. 7210) and ?-smooth muscle actin (cat. no. 53142); HRP-conjugated goat anti-rabbit and rabbit anti-goat IgG were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Primary antibodies to HIF-1? (cat. no. 2185) and VEGF-A (cat. no.
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