SoRi Jang

Full Length Guangbo Chen PhD, Guangbo Chen PhD orcid.org/0000-0003-2179-1174 Institute for Immunity, Transplantation and Infection, School of Medicine, Stanford University, Stanford, CA, USA Search for more papers by this author Gail H. Deutsch MD, Gail H.

Abstract Although much is known about the biochemical regulation of glycolytic enzymes, less is understood about how they are organized inside cells. We systematically examine the dynamic subcellular localization of glycolytic protein phosphofructokinase-1/PFK-1.1 in Caenorhabditis elegans. We determine that endogenous PFK-1.1 localizes to subcellular compartments in vivo. In neurons, PFK-1.1 forms phase-separated condensates near synapses in response to energy stress from transient hypoxia.

Highlights • A metabolic compartment forms in vivo near synapses to meet local energy demands • Under energy stress, glycolytic proteins redistribute to form clusters at synapses • The glycolytic metabolon is needed for the synaptic vesicle cycle • Disruption of glycolytic metabolon impairs synaptic recovery and affects locomotion Summary Changes in neuronal activity create local and transient changes in energy demands at synapses.