Abstract In the present study, we analyzed the role played by the apoplast in the crosstalk between biotic and abiotic stress conditions. In particular, we studied the crosstalk between nitrogen (N) limitation and infection of the model plant Arabidopsis thaliana by E. amylovora, an apoplastic bacterium. Our previous findings indicated that low N (LN) conditions increase E. amylovora in planta titers and expression of virulence factors.