Takayoshi Matsubara

Fucci fluorescent ubiquitination-based cell cycle indicator G1 Gap 1 phase G2 Gap 2 phase HCA high-content imaging analysis M mitotic phase mAG monomeric Azami-Green mKO2 monomeric Kusabira-Orange2 ROI region of interest S synthesis phase 1 INTRODUCTION Bioactive compounds exert some effect on living cells, causing phenotypic changes. Among these, those that are found to affect the proliferation and viability of cancer cells may become anticancer agents.

Highlights • Cerebral organoids can recapitulate cerebral characters in 3D order • A functional neural network was efficiently formed after dissociation of organoids • Calcium activity patterns were examined with clustering and the cell distribution • A powerful system for the functional analysis of human neuronal networks Summary The cerebrum is a major center for brain function, and its activity is derived from the assembly of activated cells in neural networks.