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Takis Biotech

As seen in: Cell Press
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Articles

Binding of steroid substrates reveals the key to the productive transition of the cytochrome P450 OleP

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By Antonella Costanzo, Francesca Fata, P.le Aldo Moro, Takis Biotech
| Cell Press Verified
Highlights • Crystal structures of OleP in complex with steroids were determined • Binding to lithocholic acid efficiently activates the closure of the OleP active site • The C17 substituent in steroids affects the selectivity of the OleP reaction Summary OleP is a bacterial cytochrome P450 involved in oleandomycin biosynthesis as it catalyzes regioselective epoxidation on macrolide intermediates.
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