Tinsley Building

Loading metrics Open Access Peer-reviewed Research Article ? This is an uncorrected proof. Citation: Ainsworth M, Galeazzi JM, Pedreira C, Stokes MG, Buckley MJ (2025) Transient frontopolar cortex stimulation induces prolonged disruption to counterfactual processing. PLoS Biol 23(11): e3003495.

In vivo Two-Photon calcium imaging – synthetic AKH bath application Functional-imaging experiments were performed as described above with some minor modifications. All flies were raised at 25 °C and 2-6 day old male and female flies were used. Flies were immobilized on ice and mounted upside down in a custom-made chamber allowing to dissect them under the proboscis in order to visualize AKHR neurons.

Highlights • Mushroom body output neurons respond with excitation to odor on- and offset • On and off responses reflect the convergence of oppositely tuned Kenyon cells (KCs) • Opponent KCs compete by eliciting inhibitory feedback from a common interneuron pool • KCs and interneurons communicate through graded potentials rather than spikes Summary The mushroom bodies of Drosophila contain circuitry compatible with race models of perceptual choice.