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Tomoji Suenaga on Muck Rack

Tomoji Suenaga

As seen in: The Journal of Biochemistry
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Articles

Charge-state-distribution analysis of Bach2 intrinsically disordered heme binding region

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By Miki Watanabe-Matsui, Hiroki Shima, Tomoji Suenaga, Tamami Uejima
| The Journal of Biochemistry Verified
Bach2 is a transcriptional repressor that plays an important role in the differentiation of T-cells and B-cells. Bach2 is functionally regulated by heme binding, and possesses five Cys-Pro Cys-Pro (CP)-motifs as the heme binding site. To reveal the molecular mechanism of heme binding by Bach2, the intrinsically disordered heme binding region (a.a. 331-520; Bach2331–520) and its CP-motif mutant were prepared and characterized with and without heme, by UV-Vis spectroscopy and thermal profiles.
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