Victoria Pappas

Get full text access Log in, subscribe or purchase for full access. References 1. Shen, L. ∙ Weber, C.R. ... Tight junction pore and leak pathways: a dynamic duo Annu. Rev. Physiol. 2011; 73:283-309 2. Farquhar, M.G. ∙ Palade, G.E. Junctional complexes in various epithelia J. Cell Biol. 1963; 17:375-412 3. Claude, P. Morphological factors influencing transepithelial permeability: a model for the resistance of the zonula occludens J. Membr. Biol. 1978; 39:219-232 4. Chalcroft, J.P. ∙ Bullivant, S.

Abstract Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens. The combination of correlative cryo-fluorescence microscopy, focused-ion-beam milling, substrate micropatterning, and MPACT supports studies that contextually define the three-dimensional architecture of cells.