Vijender Chaitankar

1 Introduction Cancer is one of the leading causes of death worldwide (Ferlay et al., 2021). Discovery of new and better ways to detect and treat cancer therefore remains an urgent global health challenge. During tumor establishment, several features important for the organs’ normal function and homeostasis are hijacked by the tumor to facilitate its growth and progression.

Publication HistoryPublished: December 7, 2017Accepted: October 26, 2017; Received in revised form: September 15, 2017; Received: July 7, 2017;

Expression cassettes of SpCas9 and sgRNA were delivered by two separate AAV vectors (Fig. 1a). The Streptococcus pyogenes Cas9 (SpCas9) coding sequence was placed in between a photoreceptor-specific human rhodopsin kinase (RK) promoter20 and a synthetic polyadenylation signal. A human RNA polymerase III promoter U6 was used to drive sgRNA expression. A tdTomato expression cassette was included in the sgRNA vector to track transduction (Fig. 1a).